PD-1 deficiency promotes TFH cells expansion in ITV-immunized mice by upregulating cytokines secretion.

ABSTRACT:

Background

T follicular helper (TFH) cells are fundamental for the development of humoral immunity. In our previous study, we found that PD-1 deficiency substantially promoted the expansion of Plasmodium-specific TFH cells and enhanced the humoral immunity of ITV (infection treatment vaccine)-immunized mice. However, the underlying mechanism by which PD-1 signaling modulates TFH cells activation remains unclear.

Methods

Mice were immunized with the ITV following the standard procedures. The activation phenotype of CD11c⁺CXCR5⁺ dendritic cells (DCs), the frequency and number of splenic follicular regulatory T cells (TFR cells), Plasmodium-specific TFH cells and germinal center (GC) B cells were analyzed by FACS. The levels of serum cytokines were quantif

SUBMITTER: Liu T 

PROVIDER: S-EPMC6035468 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature