Distinct immune signatures in directly treated and distant tumors result from TLR adjuvants and focal ablation.

ABSTRACT: Both adjuvants and focal ablation can alter the local innate immune system and trigger a highly effective systemic response. Our goal is to determine the impact of these treatments on directly treated and distant disease and the mechanisms for the enhanced response obtained by combinatorial treatments. Methods: We combined RNA-sequencing, flow cytometry and TCR-sequencing to dissect the impact of immunotherapy and of immunotherapy combined with ablation on local and systemic immune components. Results: With administration of a toll-like receptor agonist agonist (CpG) alone or CpG combined with same-site ablation, we found dramatic differences between the local and distant tumor environments, where the directly treated tumors were skewed to high expression of F4/80,

SUBMITTER: Chavez M 

PROVIDER: S-EPMC6037035 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature