Metabolic engineering of E. coli for the production of O-succinyl-l-homoserine with high yield.

ABSTRACT: O-succinyl-l-homoserine (OSH) is a promising platform chemical for the production of C4 chemicals with huge market potential which can be produced by fermentation from glucose. To construct a strain capable of producing OSH with high yield, the metJ (encodes transcriptional repressor) and metI (encodes a subunit of dl-methionine transporter) were deleted in Escherichia coli W3110 to obtain a strain E. coli ∆JI. Then, overexpression of metL (encodes bifunctional aspartate kinase/homoserine dehydrogenase II) and inactivation of metB (encodes cystathionine γ-synthase) were implemented in one step, and the OSH titer of the resulting strain E. coli ∆JIB^* TrcmetL was dramatically increased to 7.30 g/L. The feedback regulat

SUBMITTER: Huang JF 

PROVIDER: S-EPMC6037649 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature