The 180 splice variant of NCAM-containing exon 18-is specifically expressed in small cell lung cancer cells.

ABSTRACT:

Background

The Neural Cell Adhesion Molecule (NCAM) is a glycoprotein expressed as 120, 140 and/or 180 kDa isoforms, all derived through alternative splicing of a single gene. NCAM 120 contains no intracellular domain, whereas NCAM 140 and 180 have different intracellular domains determined by alternative splicing of exon 18. NCAM has been described as a biomarker to discriminate small cell lung cancer (SCLC) from non-SCLC (NSCLC). However, peripheral blood mononuclear cells (PBMC) also express NCAM. We studied the expression of NCAM splice variants in cell lines, tumor tissues and control cells.

Methods

Using reverse transcriptase-PCR we evaluated the expression of NCAM exon 18 splice variants in lung cancers cell lines, control cell lines, PBMC of healthy controls and SCLC

SUBMITTER: Vander Borght A 

PROVIDER: S-EPMC6037973 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature