Rapid and Scalable Preparation of Bacterial Lysates for Cell-Free Gene Expression.

ABSTRACT: Cell-free gene expression systems are emerging as an important platform for a diverse range of synthetic biology and biotechnology applications, including production of robust field-ready biosensors. Here, we combine programmed cellular autolysis with a freeze-thaw or freeze-dry cycle to create a practical, reproducible, and a labor- and cost-effective approach for rapid production of bacterial lysates for cell-free gene expression. Using this method, robust and highly active bacterial cell lysates can be produced without specialized equipment at a wide range of scales, making cell-free gene expression easily and broadly accessible. Moreover, live autolysis strain can be freeze-dried directly and subsequently lysed upon rehydration to produce active lysate. We demonstrate the utility of au

SUBMITTER: Didovyk A 

PROVIDER: S-EPMC6038143 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature