Evidence for histidine-rich protein 2 immune complex formation in symptomatic patients in Southern Zambia.

ABSTRACT:

Background

Rapid diagnostic tests based on histidine-rich protein 2 (HRP2) detection are the primary tools used to detect Plasmodium falciparum malaria infections. Recent conflicting reports call into question whether α-HRP2 antibodies are present in human host circulation and if resulting immune complexes could interfere with HRP2 detection on malaria RDTs. This study sought to determine the prevalence of immune-complexed HRP2 in a low-transmission region of Southern Zambia.

Methods

An ELISA was used to quantify HRP2 in patient sample DBS extracts before and after heat-based immune complex dissociation. A pull-down assay reliant on proteins A, G, and L was developed and applied for IgG and IgM capture and subsequent immunoprecipitation of any HRP2 present in immune complexe

SUBMITTER: Markwalter CF 

PROVIDER: S-EPMC6038308 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature