A "top-down" approach to actuate poly(amine-co-ester) terpolymers for potent and safe mRNA delivery.

ABSTRACT: Gene delivery is known to be a complicated multi-step biological process. It has been observed that subtle differences in the structure and properties of polymeric materials used for gene delivery can lead to dramatic differences in transfection efficiency. Therefore, screening of properties is pivotal to optimizing the polymer. So far, most polymeric materials are built in a "bottom-up" manner, i.e. synthesized from monomers that allow modification of polymer composition or structural factors. With this method, we previously synthesized and screened a library of biodegradable poly(amine-co-ester) (PACE) terpolymers for optimized DNA delivery. However, it can be tedious and time consuming to synthesize a polymer library for screening, particularly when small changes of a factor need to be

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC6038928 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature