Condensin ATPase motifs contribute differentially to the maintenance of chromosome morphology and genome stability.

ABSTRACT: Effective transfer of genetic information during cell division requires a major reorganization of chromosome structure. This process is triggered by condensin, a conserved pentameric ATPase essential for chromosome condensation. How condensin harnesses the energy of ATP hydrolysis to promote chromatin reorganization is unknown. To address this issue, we performed a genetic screen specifically focused on the ATPase domain of Smc4, a core subunit of condensin. Our screen identified mutational hotspots that impair condensin's ability to condense chromosomes to various degrees. These mutations have distinct effects on viability, genome stability, and chromosome morphology, revealing unique thresholds for condensin enzymatic activity in the execution of its cellular functions. Biochemical analy

SUBMITTER: Palou R 

PROVIDER: S-EPMC6039025 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature