Cellomics approach for high-throughput functional annotation of Caenorhabditis elegans neural network.

ABSTRACT: In Caenorhabditis elegans, which has only 302 neurons, relationships between behaviors and neural networks are not easily elucidated. In this study, we proposed a novel cellomics approach enabling high-throughput and comprehensive exploration of the functions of a single neuron or a subset of neurons in a complex neural network on a particular behavior. To realize this, we combined optogenetics and Brainbow technologies. Using these technologies, we established a C. elegans library where opsin is labeled in a randomized pattern. Behavioral analysis on this library under light illumination enabled high-throughput annotation of neurons affecting target behaviors. We applied this approach to the egg-laying behavior of C. elegans and succeeded in high-throughput confirmation that hermaphrodite

SUBMITTER: Aoki W 

PROVIDER: S-EPMC6039433 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature