Antibody targeting of a specific region of <i>Pfs47</i> blocks <i>Plasmodium falciparum</i> malaria transmission.

ABSTRACT: Transmission-blocking vaccines are based on eliciting antibody responses in the vertebrate host that disrupt parasite development in the mosquito vector and prevent malaria transmission. The surface protein Pfs47 is present in Plasmodium falciparum gametocytes and female gametes. The potential of Pfs47 as a vaccine target was evaluated. Soluble full-length recombinant protein, consisting of three domains, was expressed in E. coli as a thioredoxin fusion (T-Pfs47). The protein was immunogenic, and polyclonal and monoclonal antibodies (mAb) were obtained, but they did not confer transmission blocking activity (TBA). All fourteen mAb targeted either domains 1 or 3, but not domain 2 (D2), and immune reactivity to D2 was also very low in polyclonal mouse IgG after T-Pfs47 immuniza

SUBMITTER: Canepa GE 

PROVIDER: S-EPMC6039440 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature