Oligomerization of a G protein-coupled receptor in neurons controlled by its structural dynamics.

ABSTRACT: G protein coupled receptors (GPCRs) play essential roles in intercellular communication. Although reported two decades ago, the assembly of GPCRs into dimer and larger oligomers in their native environment is still a matter of intense debate. Here, using number and brightness analysis of fluorescently labeled receptors in cultured hippocampal neurons, we confirm that the metabotropic glutamate receptor type 2 (mGlu₂) is a homodimer at expression levels in the physiological range, while heterodimeric GABA_B receptors form larger complexes. Surprisingly, we observed the formation of larger mGlu₂ oligomers upon both activation and inhibition of the receptor. Stabilizing the receptor in its inactive conformation using biochemical constraints also led to the obse

SUBMITTER: Moller TC 

PROVIDER: S-EPMC6039492 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature