Oxidative degradation of polyamines by serum supplement causes cytotoxicity on cultured cells.

ABSTRACT: Serum is a common supplement for cell culture due to it containing the essential active components for the growth and maintenance of cells. However, the knowledges of the active components in serum are incomplete. Apart from the direct influence of serum components on cultured cells, the reaction of serum components with tested drugs cannot be ignored, which usually results in the false conclusion on the activity of the tested drugs. Here we report the toxicity effect of polyamines (spermidine and spermine) on cultured cells, especially on drug-resistant cancer cell lines, which resulted from the oxidative degradation of polyamines by amine oxidases in serum supplement. Upon adding spermidine or spermine, high concentration of H₂O_2, an enzyme oxidation product of poly

SUBMITTER: Wang L 

PROVIDER: S-EPMC6039494 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature