Evaluating lipid mediator structural complexity using ion mobility spectrometry combined with mass spectrometry.

ABSTRACT: AIM:Lipid mediators (LMs) are broadly defined as a class of bioactive lipophilic molecules that regulate cell-to-cell communication events with many having a strong correlation with various human diseases and conditions. LMs are usually analyzed with  LC-MS, but their numerous isomers greatly complicate the measurements with essentially identical fragmentation spectra and LC separations are not always sufficient for distinguishing the features. Results/methodology: In this work, we characterized LMs using ion mobility spectrometry (IMS) coupled with MS (IMS-MS). The collision cross-sections and m/z values from the IMS and MS analyses displayed distinct trend lines. Specifically, the structural trend lines for sodiated LMs originating from docosahexaenoic acid had the smallest collision cro

SUBMITTER: Kyle JE 

PROVIDER: S-EPMC6040087 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature