Kindlin supports platelet integrin αIIbβ3 activation by interacting with paxillin.

ABSTRACT: Kindlins play an important role in supporting integrin activation by cooperating with talin; however, the mechanistic details remain unclear. Here, we show that kindlins interacted directly with paxillin and that this interaction could support integrin αIIbβ3 activation. An exposed loop in the N-terminal F₀ subdomain of kindlins was involved in mediating the interaction. Disruption of kindlin binding to paxillin by structure-based mutations significantly impaired the function of kindlins in supporting integrin αIIbβ3 activation. Both kindlin and talin were required for paxillin to enhance integrin activation. Interestingly, a direct interaction between paxillin and the talin head domain was also detectable. Mechanistically, paxillin, together with kindlin, was able to promote th

SUBMITTER: Gao J 

PROVIDER: S-EPMC6040092 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature