Natural Transformation in Vibrio parahaemolyticus: a Rapid Method To Create Genetic Deletions.

ABSTRACT: The Gram-negative bacterium Vibrio parahaemolyticus is an opportunistic human pathogen and the leading cause of seafood-borne acute gastroenteritis worldwide. Recently, this bacterium was implicated as the etiologic agent of a severe shrimp disease with consequent devastating outcomes to shrimp farming. In both cases, acquisition of genetic material via horizontal transfer provided V. parahaemolyticus with new virulence tools to cause disease. Dissecting the molecular mechanisms of V. parahaemolyticus pathogenesis often requires manipulating its genome. Classically, genetic deletions in V. parahaemolyticus are performed using a laborious, lengthy, multistep process. Here, we describe a fast and efficient method to edit this bacterium's genome based on V. para

SUBMITTER: Chimalapati S 

PROVIDER: S-EPMC6040184 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature