Effect of Freeze-Thaw on a Midtemperate Soil Bacterial Community and the Correlation Network of Its Members.

ABSTRACT: Freeze-thaw (FT) events can influence soil functions. However, the overall impact of FTs on soil bacterial communities, especially in temperate regions, remains unclear. In this study, soil samples were collected from a midtemperate region in the northeast of China, and three incubation tests were then designed with varied FT amplitudes (i.e., at a freezing temperature of -15, -9, and -3°C, respectively), frequencies of FT cycles (i.e., under one, six, and 15 FT cycles, respectively) and soil water content (SWC) values (i.e., at 10 and 30% SWC, respectively). High-throughput sequencing of 16S rRNA gene amplicons was performed and the functional profile was further predicted based on these data, in addition to examinations of bulk microbial properties. Data analyses suggested that, first of

SUBMITTER: Juan Y 

PROVIDER: S-EPMC6040300 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature