Effect of clinical parameters on the ocular surface microbiome in children and adults.

ABSTRACT:

Purpose

To perform a pilot study to characterize the effect of clinical parameters on the ocular surface microbiome (OSM) in children and adults using 16s ribosomal RNA sequencing.

Methods

Prospective, cross-sectional study using 16s sequencing to evaluate the OSM. Comparisons were made in bacterial composition by 1) age, 2) gender, 3) sampling location of the ocular and periocular surfaces, and 4) topical drop use. 16s sequencing was performed using Illumina MiSeq 250 and analyzed using Qiime.

Results

Thirty patients (15 children [mean 3.7 years], 15 adults [mean 60.4 years]) were sampled. Both principal coordinate analysis and unifrac distance analysis showed significant differences in the composition between the pediatric and adult OSMs (both p=0.001). The e

SUBMITTER: Cavuoto KM 

PROVIDER: S-EPMC6040630 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature