Three-dimensional behavioural phenotyping of freely moving C. elegans using quantitative light field microscopy.

ABSTRACT: Behavioural phenotyping of model organisms is widely used to investigate fundamental aspects of organism biology, from the functioning of the nervous system to the effects of genetic mutations, as well as for screening new drug compounds. However, our capacity to observe and quantify the full range and complexity of behavioural responses is limited by the inability of conventional microscopy techniques to capture volumetric image information at sufficient speed. In this article we describe how combining light field microscopy with computational depth estimation provides a new method for fast, quantitative assessment of 3D posture and movement of the model organism Caenorhabditis elegans (C. elegans). We apply this technique to compare the behaviour of cuticle collagen mutants, finding sign

SUBMITTER: Shaw M 

PROVIDER: S-EPMC6040744 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature