A low-cost microwell device for high-resolution imaging of neurite outgrowth in 3D.

ABSTRACT:

Objective

Current neuronal cell culture is mostly performed on two-dimensional (2D) surfaces, which lack many of the important features of the native environment of neurons, including topographical cues, deformable extracellular matrix, and spatial isotropy or anisotropy in three dimensions. Although three-dimensional (3D) cell culture systems provide a more physiologically relevant environment than 2D systems, their popularity is greatly hampered by the lack of easy-to-make-and-use devices. We aim to develop a widely applicable 3D culture procedure to facilitate the transition of neuronal cultures from 2D to 3D.

Approach

We made a simple microwell device for 3D neuronal cell culture that is inexpensive, easy to assemble, and fully compatible with commonly used imaging techn

SUBMITTER: Ren Y 

PROVIDER: S-EPMC6040821 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature