Extracellular matrix scaffold and hydrogel derived from decellularized and delipidized human pancreas.

ABSTRACT: Extracellular matrix (ECM) plays an important developmental role by regulating cell behaviour through structural and biochemical stimulation. Tissue-specific ECM, attained through decellularization, has been proposed in several strategies for tissue and organ replacement. Decellularization of animal pancreata has been reported, but the same methods applied to human pancreas are less effective due to higher lipid content. Moreover, ECM-derived hydrogels can be obtained from many decellularized tissues, but methods have not been reported to obtain human pancreas-derived hydrogel. Using novel decellularization methods with human pancreas we produced an acellular, 3D biological scaffold (hP-ECM) and hydrogel (hP-HG) amenable to tissue culture, transplantation and proteomic applications. The in

SUBMITTER: Sackett SD 

PROVIDER: S-EPMC6041318 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature