DCE-MRI of Sunitinib-Induced Changes in Tumor Microvasculature and Hypoxia: A Study of Pancreatic Ductal Adenocarcinoma Xenografts.

ABSTRACT: The purpose of this study was dual: to investigate (a) whether sunitinib may induce changes in tumor microvasculature and hypoxia in pancreatic ductal adenocarcinoma (PDAC) and (b) whether any changes can be detected by DCE-MRI. Sunitinib-treated and untreated control tumors of two PDAC xenograft models (BxPC-3 and Panc-1) were subjected to DCE-MRI before the imaged tumors were prepared for quantitative analysis of immunohistochemical preparations. Pimonidazole was used as a hypoxia marker, and fraction of hypoxic tissue (HF_Pim), density of CD31-positive microvessels (MVD_CD31), and density of αSMA-positive microvessels (MVD_αSMA) were measured. Parametric images of K^trans and v_e were derived from the DCE-MRI data by using the Tofts phar

SUBMITTER: Wegner CS 

PROVIDER: S-EPMC6041378 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature