Selective protein recognition in supported lipid bilayer arrays by tailored, dual-mode deep cavitand hosts.

ABSTRACT: Self-folding deep cavitands with variably functionalized upper rims are able to selectively immobilize proteins at a biomimetic supported lipid bilayer surface. The immobilization process takes advantage of the dual-mode binding capabilities of the hosts, combining a defined binding pocket with upper rim charged/H-bonding groups. A variety of proteins can be selectively immobilized at the bilayer interface, either via complementary charge/H-bonding interactions, cavity-based molecular recognition, or a combination of both. The immobilization process can be used to bind unmodified native proteins, epitopes for bioadhesion, or proteins covalently modified with suitable RNMe₃⁺ binding "handles" and charged groups that can either match or mismatch with the cavitand rim. T

SUBMITTER: Perez L 

PROVIDER: S-EPMC6041475 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature