Different-Length Hydrazone Activated Polymers for Plasmid DNA Condensation and Cellular Transfection.

ABSTRACT: The recent advances in genetic engineering demand the development of conceptually new methods to prepare and identify efficient vectors for the intracellular delivery of different nucleotide payloads ranging from short single-stranded oligonucleotides to larger plasmid double-stranded circular DNAs. Although many challenges still have to be overcome, polymers hold great potential for intracellular nucleotide delivery and gene therapy. We here develop and apply the postpolymerization modification of polyhydrazide scaffolds, with different degree of polymerization, for the preparation of amphiphilic polymeric vehicles for the intracellular delivery of a circular plasmid DNA. The hydrazone formation reactions with a mixture of cationic and hydrophobic aldehydes proceed in physiologically comp

SUBMITTER: Priegue JM 

PROVIDER: S-EPMC6041776 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature