Ex vivo model of epilepsy in organotypic slices-a new tool for drug screening.

ABSTRACT:

Background

Epilepsy is a prevalent neurological disorder worldwide. It is characterized by an enduring predisposition to generate seizures and its development is accompanied by alterations in many cellular processes. Organotypic slice cultures represent a multicellular environment with the potential to assess biological mechanisms, and they are used as a starting point for refining molecules for in vivo studies. Here, we investigated organotypic slice cultures as a model of epilepsy.

Methods

We assessed, by electrophysiological recordings, the spontaneous activity of organotypic slices maintained under different culture protocols. Moreover, we evaluated, through molecular-based approaches, neurogenesis, neuronal death, gliosis, expression of proinflammatory cytokines, and ac

SUBMITTER: Magalhaes DM 

PROVIDER: S-EPMC6042335 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature