Stability of important antibodies for kidney disease: pre-analytic methodological considerations.

ABSTRACT:

Background

The importance of circulating antibodies as biomarkers of kidney disease has recently been recognized. However, no study has systematically described the methodology of sample preparation and storage regarding antibodies as biomarkers of kidney disease. It remains unknown whether repetitive freeze-thaw cycles, physical disturbances, storage at different temperatures or for different periods of time, or haemolytic or turbid serum samples affect antibody measurements. The aim of this study was to investigate the stabilities of antibodies associated with kidney disease in serum samples under various relevant clinical and research conditions.

Methods

We stored serum samples in the following different conditions: repetitive freeze-thaw cycles (1, 6 or 12 times), long-t

SUBMITTER: Han Q 

PROVIDER: S-EPMC6042478 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature