Computer Analysis of Glioma Transcriptome Profiling: Alternative Splicing Events.

ABSTRACT: Here we present the analysis of alternative splicing events on an example of glioblastoma cell culture samples using a set of computer tools in combination with database integration. The gene expression profiles of glioblastoma were obtained from cell culture samples of primary glioblastoma which were isolated and processed for RNA extraction. Transcriptome profiling of normal brain samples and glioblastoma were done by Illumina sequencing. The significant differentially expressed exon-level probes and their corresponding genes were identified using a combination of the splicing index method. Previous studies indicated that tumor-specific alternative splicing is important in the regulation of gene expression and corresponding protein functions during cancer development. Multiple alternativ

SUBMITTER: Babenko VN 

PROVIDER: S-EPMC6042819 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature