Molecular and functional characterization of the adiponectin (AdipoQ) gene in goat skeletal muscle satellite cells.

ABSTRACT:

Objective

It is commonly accepted that adiponectin binds to its two receptors to regulate fatty acid metabolism in adipocytes. To better understand their functions in the regulation of intramuscular adipogenesis in goats, we cloned the three genes (adiponectin [AdipoQ], adiponectin receptor 1 [AdipoR1], and AdipoR2) encoding these proteins and detected their mRNA distribution in different tissues. We also determined the role of AdipoQ in the adipogenic differentiation of goat skeletal muscle satellite cells (SMSCs).

Methods

SMSCs were isolated using 1 mg/mL Pronase E from the longissimus dorsi muscles of 3-day-old female Nanjiang brown goats. Adipogenic differentiation was induced in satellite cells by transferring the cells to Dulbecco's modified Eagle's medium su

SUBMITTER: Wang L 

PROVIDER: S-EPMC6043445 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature