Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery.

ABSTRACT: The emerging discipline of bacterial glycoengineering has made it possible to produce designer glycans and glycoconjugates for use as vaccines and therapeutics. Unfortunately, cell-based production of homogeneous glycoproteins remains a significant challenge due to cell viability constraints and the inability to control glycosylation components at precise ratios in vivo. To address these challenges, we describe a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation. This technology leverages a glyco-optimized Escherichia coli strain to source cell extracts that are selectively enriched with glycosylation components, including oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides

SUBMITTER: Jaroentomeechai T 

PROVIDER: S-EPMC6043479 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature