Systematic RNA-interference in primary human monocyte-derived macrophages: A high-throughput platform to study foam cell formation.

ABSTRACT: Macrophage-derived foam cells are key regulators of atherogenesis. They accumulate in atherosclerotic plaques and support inflammatory processes by producing cytokines and chemokines. Identifying factors that regulate macrophage lipid uptake may reveal therapeutic targets for coronary artery disease (CAD). Here, we establish a high-throughput screening workflow to systematically identify genes that impact the uptake of DiI-labeled low-density lipoprotein (LDL) into monocyte-derived primary human macrophages. For this, monocytes isolated from peripheral blood were seeded onto 384-well plates, solid-phase transfected with siRNAs, differentiated in vitro into macrophages, and LDL-uptake per cell was measured by automated microscopy and quantitative image analysis. We applied this workflow to

SUBMITTER: Domschke G 

PROVIDER: S-EPMC6043567 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature