Rat astrocytes during anoxia: Secretome profile of cytokines and chemokines.

ABSTRACT:

Introduction

The precise mechanisms of the inflammatory responses after cerebral ischemia in vivo are difficult to elucidate because of the complex nature of multiple series of interactions between cells and molecules. This study explored temporal patterns of secretion of 30 cytokines and chemokines from Sprague Dawley rat astrocytes in primary culture in order to elucidate signaling pathways that are triggered by astrocytes during anoxia.

Methods

Primary cultures of rat brain astrocytes were incubated for periods of 2-24 hr in the absence of oxygen (anoxia) or under normal partial pressure of oxygen (controls). Simultaneous detection of 29 cytokines and chemokines in the samples was performed using a rat cytokine array panel, while the temporal pattern of angiopoietin-1 (An

SUBMITTER: Samy ZA 

PROVIDER: S-EPMC6043693 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature