In Silico Selection Approach to Develop DNA Aptamers for a Stem-like Cell Subpopulation of Non-small Lung Cancer Adenocarcinoma Cell Line A549.

ABSTRACT:

Background

Detection of circulating lung cancer cells with cancer-stem like characteristics would represent an improved tool for disease prognosis. However, current antibodies based methods have some disadvantages and therefore cell SELEX (Systematic Evolution of Ligands by Exponential Enrichment) was used to develop DNA aptamers, recognizing cell surface markers of non-small lung carcinoma (NSLC) cells.

Materials and methods

The human adenocarcinoma cell line A549 was used for selection in seven cell SELEX cycles. We used human blood leukocytes for negative selection, and lung stem cell protein marker CD90 antibody binding A549 cells for positive selection.

Results

The obtained oligonucleotide sequences after the seventh SELEX cycle were subjected to in silico

SUBMITTER: Vidic M 

PROVIDER: S-EPMC6043879 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature