Key role of piRNAs in telomeric chromatin maintenance and telomere nuclear positioning in Drosophila germline.

ABSTRACT:

Background

Telomeric small RNAs related to PIWI-interacting RNAs (piRNAs) have been described in various eukaryotes; however, their role in germline-specific telomere function remains poorly understood. Using a Drosophila model, we performed an in-depth study of the biogenesis of telomeric piRNAs and their function in telomere homeostasis in the germline.

Results

To fully characterize telomeric piRNA clusters, we integrated the data obtained from analysis of endogenous telomeric repeats, as well as transgenes inserted into different telomeric and subtelomeric regions. The small RNA-seq data from strains carrying telomeric transgenes demonstrated that all transgenes belong to a class of dual-strand piRNA clusters; however, their capacity to produce piRNAs varies significantly

SUBMITTER: Radion E 

PROVIDER: S-EPMC6043984 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature