Construction of a trifunctional cellulase and expression in Saccharomyces cerevisiae using a fusion protein.

ABSTRACT:

Background

Cellulose is the most important component of lignocellulose, and its degradation requires three different types of enzymes to act synergistically. There have been reports of single gene duality, but no gene has been described to have more than two functions. Cloning and expression of fusion cellulases containing more than two kinds of catalytic domains has not been reported thus far.

Results

We synthesized three different cellulase genes and linked the three catalytic domains with a (G₄S)₃ flexible linker. The trifunctional cellulase gene (BCE) containing three types of cellulase functions was constructed and expressed in S. cerevisiae successfully. The β-glucosidase, the exoglucanase and the endoglucanase activity of the trifunctional cellul

SUBMITTER: Liu ZL 

PROVIDER: S-EPMC6044064 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature