Catecholamine Detection Using a Functionalized Poly(l-dopa)-Coated Gate Field-Effect Transistor.

ABSTRACT: A highly sensitive catecholamine (CA) sensor was created using a biointerface layer composed of a biopolymer and a potentiometric detection device. For the detection of CAs, 3-aminophenylboronic acid (3-NH₂-PBA) was reacted with the carboxyl side chain of l-3,4-dihydroxyphenylalanine (l-dopa, LD) and the PBA-modified l-dopa was directly copolymerized with LD on an Au electrode, resulting in a 3.5 nm thick PBA-modified poly(PBA-LD/LD) layer-coated Au electrode. By connecting the PBA-LD-coated Au electrode to a field-effect transistor (FET), the molecular charge changes at the biointerface of the Au electrode, which was caused by di-ester binding of the PBA-CA complex, were transduced into gate surface potential changes. Effective CAs included LD, dopamine (DA), norepinephrine (NE

SUBMITTER: Kajisa T 

PROVIDER: S-EPMC6044613 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature