Class I Polyhydroxyalkanoate Synthase from the Purple Photosynthetic Bacterium Rhodovulum sulfidophilum Predominantly Exists as a Functional Dimer in the Absence of a Substrate.

ABSTRACT: Polyhydroxyalkanoates (PHAs) are a family of biopolyesters that accumulate as carbon and energy storage compounds in a variety of micro-organisms. The marine purple photosynthetic bacterium Rhodovulum sulfidophilum is capable of synthesizing PHA. In this study, we cloned a gene encoding a class I PHA synthase from R. sulfidophilum (phaC_Rs ) and synthesized PhaC_Rs using a cell-free protein expression system. The specific activity of PhaC_Rs increased linearly as the (R)-3-hydroxybutyryl-coenzyme A (3HB-CoA) concentration increased and never reached a plateau, even at 3.75 mM 3HB-CoA, suggesting that PhaC_Rs was not saturated because of low substrate affinity. Size exclusion chromatography and native polyacrylamide

SUBMITTER: Higuchi-Takeuchi M 

PROVIDER: S-EPMC6044645 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature