Synthesis of Calcium Bisphosphonate/Calcium Polyacrylate Spheres for Gene Delivery.

ABSTRACT: Calcium bisphosphonate/calcium polyacrylate spheres were synthesized by a facile method and applied for the first time as gene vectors for transfection. The colloidal spheres of the PAA-Ca²⁺-H₂O complex, formed by sodium polyacrylate and calcium ions in the solution, were used as template to synthesize a spherical PAA-Ca²⁺-BPMP composite (CaBPMP/CaPAA) in the presence of 1,4-bis(phosphomethyl)piperazine (BPMP). The CaBPMP/CaPAA composite exhibits uniform and well-dispersed spheres with a particle size of about 200 nm as expected. The cytotoxicity assays confirm that CaBPMP/CaPAA spheres are quite safe for different cells even at a high concentration of 500 μg/mL. In vitro transfection results show that CaBPMP/CaPAA spheres serving as gene vectors are capabl

SUBMITTER: Wei X 

PROVIDER: S-EPMC6044815 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature