TMPyP Inhibits Amyloid-? Aggregation and Alleviates Amyloid-Induced Cytotoxicity.
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ABSTRACT: The aggregation or misfolding of amyloid-? (A?) is a major pathological hallmark of Alzheimer's disease (AD). The regulation of A? aggregation is thought to be an effective strategy for AD treatment. The capability of a water-soluble porphyrin, 5,10,15,20-tetrakis(N-methyl-4-pyridyl)porphyrin (TMPyP), to inhibit A? aggregation and to lower A?-induced toxicity was demonstrated. As evidenced by surface plasmon resonance and circular dichroism, TMPyP can not only disrupt A? aggregation but also disassemble the preformed A? aggregates. The atomic force microscopy imaging proves that TMPyP inhibits the formation of both oligomers and fibrils. Molecular dynamic simulations provide an insight into the interaction between TMPyP and A? at the molecular level. The half-maximal inhibitory concentrations of TMPyP acting on the oligomers and fibrils were determined to be 0.6 and 0.43 ?M, respectively. As a member of porphyrin family, TMPyP is of rather low cytotoxicity, and the cytotoxicity of the A? aggregates was also relieved upon coincubation with TMPyP. The excellent performance of TMPyP thus makes it a potential drug candidate for AD therapy.
SUBMITTER: Fan Y
PROVIDER: S-EPMC6044923 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
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