Infrared Nanospectroscopy of Phospholipid and Surfactin Monolayer Domains.

ABSTRACT: A main challenge in understanding the structure of a cell membrane and its interactions with drugs is the ability to chemically study the different molecular species on the nanoscale. We have achieved this for a model system consisting of mixed monolayers (MLs) of the biologically relevant phospholipid 1,2-distearoyl-sn-glycero-phosphatidylcholine and the antibiotic surfactin. By employing nano-infrared (IR) microscopy and spectroscopy in combination with atomic force microscopy imaging, it was possible to identify and chemically detect domain formation of the two constituents as well as to obtain IR spectra of these species with a spatial resolution on the nanoscale. A novel method to enhance the near-field imaging contrast of organic MLs by plasmon interferometry is proposed and d

SUBMITTER: Kastner B 

PROVIDER: S-EPMC6044929 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature