Maltose-Functionalized Hydrophilic Magnetic Nanoparticles with Polymer Brushes for Highly Selective Enrichment of N-Linked Glycopeptides.

ABSTRACT: Efficient enrichment glycoproteins/glycopeptides from complex biological solutions are very important in the biomedical sciences, in particular biomarker research. In this work, the high hydrophilic polyethylenimine conjugated polymaltose polymer brushes functionalized magnetic Fe₃O₄ nanoparticles (NPs) denoted as Fe₃O₄-PEI-pMaltose were designed and synthesized via a simple two-step modification. The obtained superhydrophilic Fe₃O₄-PEI-pMaltose NPs displayed outstanding advantages in the enrichment of N-linked glycopeptides, including high selectivity (1:100, mass ratios of HRP and bovine serum albumin (BSA) digest), low detection limit (10 fmol), large binding capacity (200 mg/g), and high enrichment recovery (above 85%).

SUBMITTER: Bi C 

PROVIDER: S-EPMC6044954 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature