Rationally Designed Probe for Reversible Sensing of Zinc and Application in Cells.

ABSTRACT: Biologically compatible fluorescent ion sensors, particularly those that are reversible, represent a key tool for answering a range of fundamental biological questions. We report a rationally designed probe with a 6'-fluoro spiropyran scaffold (5) for the reversible sensing of zinc (Zn²⁺) in cells. The 6'-fluoro substituent overcomes several limitations normally associated with spiropyran-based sensors to provide an improved signal-to-background ratio and faster photoswitching times in aqueous solution. In vitro studies were performed with 5 and the 6'-nitro analogues (6) in HEK 293 and endothelial cells. The new spiropyran (5) can detect exogenous Zn²⁺ inside both cell types and without affecting the proliferation of endothelial cells. Stu

SUBMITTER: Heng S 

PROVIDER: S-EPMC6044982 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature