Magnetic Separation of Autophagosomes from Mammalian Cells Using Magnetic-Plasmonic Hybrid Nanobeads.

ABSTRACT: Developments in subcellular fractionation strategies have provided the means to analyze the protein and lipid composition of organelles by proteomics. Here, we developed ultrasmall magnetic-plasmonic hybrid nanobeads and applied them to the isolation of autophagosomes by applying a magnetic field. The beads were chemically synthesized and comprised an Ag/FeCo/Ag core/shell/shell structure with a mean diameter of 15 nm. The Ag core and the FeCo shell conferred imaging and magnetic separation capabilities, respectively. The nanobeads were transfected into mammalian cells by lipofection. Thirty minutes after lipofection, the nanobeads colocalized with Vps26 and subsequently with LC3. Cell lysates were prepared at the appropriate time points and were subjected to magnetic separation. The separ

SUBMITTER: Takahashi M 

PROVIDER: S-EPMC6044991 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature