Probing Multiple Binding Modes of DNA Hybridization: A Comparison between Single-Molecule Observations and Ensemble Measurements.

ABSTRACT: Interactions between biomolecules are generally analyzed by ensemble measurements, assuming that the interactions occur in a single binding manner. However, such interactions may occur via multiple binding modes. We investigated the kinetics of DNA hybridization as a multiple dynamic model of biomolecular interactions. Two kinetic analyses were performed with a single-molecule observation using total internal reflection fluorescence microscopy (TIRFM) and with ensemble measurements using a quartz-crystal microbalance (QCM) biosensor. We observed the DNA hybridization of 8 and 12 bp DNAs with random sequences and dA₁₂-dT₁₂ and calculated the kinetic parameters, including the dissociation rate constant (k_off). Hybridization of 8 bp DNA proceeded mainly

SUBMITTER: Yazawa K 

PROVIDER: S-EPMC6045368 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature