Studying DNA G-Quadruplex Aptamer by ¹⁹F NMR.

ABSTRACT: In this study, we demonstrated that ¹⁹F NMR can be used to study the thrombin-binding aptamer (TBA) DNA G-quadruplex, widely used as a model structure for studying G-quadruplex aptamers. We systematically examined the structural feature of the TBA G-quadruplex aptamer with fluorine-19 (¹⁹F) labels at all of the thymidine positions. We successfully observed the structural change between the G-quadruplex and the unstructured single strand by ¹⁹F NMR spectroscopy. The thermodynamic parameters of these DNA G-quadruplex aptamers were also determined from the ¹⁹F NMR signals. We further showed that the ¹⁹F NMR method can be used to observe the complex formed by TBA G-quadruplex and thrombin. Our results suggest that ¹⁹F NMR spectr

SUBMITTER: Ishizuka T 

PROVIDER: S-EPMC6045382 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature