Single Platform for Gene and Protein Expression Analyses Using Luminescent Gold Nanoclusters.

ABSTRACT: A single platform for gene and protein expression studies is proposed to pursue rapid diagnostics. A common method to synthesize gold (Au) nanoclusters on both DNA and protein template was developed using a benchtop device. The method of synthesis is rapid and versatile and can be applied to different classes of DNA/protein. Employing luminescent Au nanoclusters as the signal-generating agents, the device enables carrying out reverse transcriptase polymerase chain reaction and array-based analyses of multiple genes/proteins simultaneously using switchable holders and custom-designed software. The device and methods were applied to evaluate gene profiling related to apoptosis in HeLa cancer cells and further to analyze the protein expressions of glutathione-S-transferase (GST) and GS

SUBMITTER: Sailapu SK 

PROVIDER: S-EPMC6045389 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature