A promoter variant of lncRNA GAS5 is functionally associated with the development of osteosarcoma.

ABSTRACT:

Background

Previous studies showed that genetic variant rs145204276 in the promoter region of GAS5 was associated with the development of human cancer including colorectal cancer and hepatocellular cancer. This study aimed to investigate the role of rs145204276 in the development of osteosarcoma (OS).

Methods

132 OS patients and 1270 healthy controls were recruited for the genotyping analysis of rs145204276. Promoter methylation level of GAS5 was determined for all patients. The tumor tissues and the adjacent normal tissue were collected from 42 patients during surgery and the relative expression of GAS5 was then quantified by Real-time PCR. The Chi-square test was used to determine the difference of genotype and allele frequency between the patients and the controls. The ge

SUBMITTER: Xu L 

PROVIDER: S-EPMC6045496 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature