Heterologous expression of Oenococcus oeni sHSP20 confers temperature stress tolerance in Escherichia coli.

ABSTRACT: Small heat shock proteins (sHSPs) are heat shock proteins sized 12-43 kDa that can protect proteins from denaturation, particularly under high temperature; sHSPs thus increase the heat tolerance capability of an organisms enabling survival in adverse climates. sHSP20 is overexpressed in Oenococcus oeni in response to low temperatures. However, we found that overexpression of sHSP20 in Escherichia coli BL21 increased the microbial survival ratio at 50 °C by almost 2 h. Adding sHSP20 to the glutamate dehydrogenase solution significantly increased the stability of the enzyme at high temperature (especially at 60-70 °C), low pH values (especially below 6.0), and high concentration of metal ions of Ga2+, Zn2+, Mn2+, and Fe3+. Notably, the coexpression of sHSP20 significantly enhanced soluble ex

SUBMITTER: Li Y 

PROVIDER: S-EPMC6045537 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature