Fate of mesoangioblasts in a vaginal birth injury model: influence of the route of administration.

ABSTRACT: Currently cell therapy is considered as an experimental strategy to assist the healing process following simulated vaginal birth injury in rats, boosting the functional and morphologic recovery of pelvic floor muscles and nerves. However, the optimal administration route and dose still need to be determined. Mesangioblasts theoretically have the advantage that they can differentiate in skeletal and smooth muscle. We investigated the fate of mesoangioblasts transduced with luciferase and green fluorescent protein reporter genes (rMABs^eGFP/fLUC) using bioluminescence, immunofluorescence and RT-PCR in rats undergoing simulated birth injury. rMABs^eGFP/fLUC were injected locally, intravenously and intra-arterially (common iliacs and aorta). Intra-arterial delivery resulted

SUBMITTER: Mori da Cunha MGMC 

PROVIDER: S-EPMC6045600 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature