Cell surface nucleolin interacts with and internalizes Bothrops asper Lys49 phospholipase A₂ and mediates its toxic activity.

ABSTRACT: Phospholipases A₂ are a major component of snake venoms. Some of them cause severe muscle necrosis through an unknown mechanism. Phospholipid hydrolysis is a possible explanation of their toxic action, but catalytic and toxic properties of PLA₂s are not directly connected. In addition, viperid venoms contain PLA₂-like proteins, which are very toxic even if they lack catalytic activity due to a critical mutation in position 49. In this work, the PLA₂-like Bothrops asper myotoxin-II, conjugated with the fluorophore TAMRA, was found to be internalized in mouse myotubes, and in RAW264.7 cells. Through experiments of protein fishing and mass spectrometry analysis, using biotinylated Mt-II as bait, we found fifteen proteins interacting with the toxin a

SUBMITTER: Massimino ML 

PROVIDER: S-EPMC6045611 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature