Codon usage clusters correlation: towards protein solubility prediction in heterologous expression systems in E. coli.

ABSTRACT: Production of soluble recombinant proteins is crucial to the development of industry and basic research. However, the aggregation due to the incorrect folding of the nascent polypeptides is still a mayor bottleneck. Understanding the factors governing protein solubility is important to grasp the underlying mechanisms and improve the design of recombinant proteins. Here we show a quantitative study of the expression and solubility of a set of proteins from Bizionia argentinensis. Through the analysis of different features known to modulate protein production, we defined two parameters based on the %MinMax algorithm to compare codon usage clusters between the host and the target genes. We demonstrate that the absolute difference between all %MinMax frequencies of the host and the target gene

SUBMITTER: Pellizza L 

PROVIDER: S-EPMC6045634 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature