Damage to the myogenic differentiation of C2C12 cells by heat stress is associated with up-regulation of several selenoproteins.

ABSTRACT: This study was conducted to profile the selenoprotein encoding genes or proteins in mouse C2C12 cells and integrate their roles in the skeletal cell damage induced by heat stress (HS). Cells were cultured at 37.0 °C or 41.5 °C for 4, 6 or 8 days. The mRNA expression of 24 selenoprotein encoding genes and abundance of 5 selenoproteins were investigated. HS suppressed myogenic differentiation and impaired the development of muscle myotubes. HS down-regulated (P < 0.01) mRNA abundance of MYOD and MYOGENIN, and decreased (P < 0.01) MYOGENIN protein expression, HS elevated (P < 0.01) HSP70 and (P < 0.01) the ratio of BCL-2 to BAX at both mRNA and protein level. Meanwhile, HS up-regulated (P < 0.01-0.05) expressions of 18, 11 and 8 selenoprotein encoding genes after 4, 6 and 8 days of hypertherm

SUBMITTER: Tang J 

PROVIDER: S-EPMC6045685 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature